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KMID : 0362220110380030189
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Journal of The Korean Research Society for Dental Materials
2011 Volume.38 No. 3 p.189 ~ p.196
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The Effect of the UV Treatment of TiO2 Nanotube Arrays on the Adhesion, Proliferation, and Osteogenic Differentiation of Human Mesenchymal Stem Cells
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Mon Kyoung-Sook
Jin Su-Hyun
Bae Ji-Myung
Oh Seung-Han
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Abstract
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We prepared ultra violet (UV) light irradiated TiO2 nanotube array on eletropolished titanium surface and estimated the effect of the UV treatment of TiO2 nanotubular structure on the adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in terms of different diameters (30 - 100 nm) of TiO2 nanotubes. Fluorescein diacetate assay resulted that the initial adhesion of hMSCs did not show any sign ifican t difference between all TiO2 nanotube diameter (p>0.05). However, the proliferation of hMSCs cultured on electropolished Ti surface were higher than those cultured on 50, 70, and 100 nm TiO2 nanotubes (p<0.05) From the results of RT-PCR assay, the values of i ntegrin β1, osteocalcin, osteoponting, and alkaline phosphatase did not show any significant difference between all experimental groups after 1 week of incubation period. However, the value of alkaline phosphatase showed the tendency of increasing
with proportional to the diameter of TiO2 nanotubes. Therefore, we can expect the UV treatment of TiO2 nanotube did not affect he adhesion, proliferation, and osteogenic differentiation of hMSCs compared to non UV treated TiO2 nanotube. Also, the enlargement of nanotube diameter is supposed to promote osteogenic differentiation of hMSCs from all results of this study.
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KEYWORD
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Electropolishing, Human mesenchymal stem cells(hMSCs), osteogenic differentiation, TiO2 nanotube, UV treatment
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